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Wright’s fixation index ( F ST ) was computed for each pairwise combination of lines based on the estimator corrected for unequal sample sizes [ 23 ] implemented in the “fsthet” package [ 24 ] in R software [ 21 ], using all SNPs that had passed the QC procedures. Then, F ST and heterozygosity averages were estimated for genomic windows of 100kb across the autosomes, with an overlap of 75kb. Putative signals of divergence were detected with two empirical strategies that are typically used in studies involving F ST tests, one that identifies the top-ranking windows [ Outlet Sale Online Clearance Outlet Store SUITS AND JACKETS Waistcoats Karl Lagerfeld 32IPMQYL7a
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] and another method that identifies outliers from the background level of F ST -heterozygosity [ 24 , 27 ]. Thus, windows were considered to show putative signatures of selection if they contained more than five SNPs, presented an average F ST within the top 1%, and outside the 95% confidence interval of their heterozygosity bin in both cross-line comparisons of NeC with one of the lines under directional selection (“NeC vs. NeS” and “NeC vs. NeT”) [see Additional file 2018 Online Buy Cheap Perfect RIPNDIP Cuffed Trousers In Camo CoOrd Purple Ripndip Limited Huge Surprise Cheap Online xrlUnjgyP
: Figure S2].

The extended haplotype homozygosity (EHH) metric is defined as the probability of randomly choosing two identical extended haplotypes that surround a specific locus [ 28 ], for which higher values are expected for selected alleles with a frequency that increased rapidly due to selection. In our study, we applied two somewhat complementary EHH-derived methods to detect signatures of selection, the cross population EHH (XP-EHH) [ 31 Phillip Lim Woman Striped Fil Coupé Silkblend Georgette Top Black Size 4 31 Phillip Lim Quality Sale Manchester Clearance Supply BkpaykRq0
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]. Whereas XP-EHH is a cross population method that is useful to detect signals of divergent selection between lines, iHS is a within-population method that allows the identification of signals of ongoing positive selection.

The XP-EHH test was applied to the comparisons between NeC and each of the lines under directional selection (“NeC vs. NeS” and “NeC vs. NeT”). XP-EHH values were averaged for 100-kb windows with an overlap of 75kb, and a cut-off threshold of 2.58 ( p <0.005) was applied to determine candidate signatures of selection. Only windows for which the average XP-EHH exceeded the upper cut-off in both cross-line comparisons and contained more than five SNPs were considered to harbor a signature of selection [see Additional file Wide Range Of Cheap Price Sapphire Pools Frill One Piece Swimsuit Blue Somedays Lovin Good Selling Cheap Online Cheap Sneakernews Free Shipping Affordable Clearance Footlocker Finishline sl4pTDB
: Figure S3], which was characterized by the presence of some high-frequency haplotypes in the NeS and NeT lines but not in the NeC line.

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No hay ningún indicador más claro de la existencia de una democracia fuerte y un estado de derecho consolidado que el respeto a la autoridad y el apego a las reglas. A menos que empecemos a respetar a las autoridades y a comportarnos de forma civilizada en nuestras interacciones con otros ciudadanos, difícilmente podremos construir una sociedad más justa e igualitaria.

La polémica que generó el incidente en el aeropuerto en que un carabinero disparó a un chofer de Uber que se negó a seguir las instrucciones y que, en cambio, intentó atropellarlo para huir del lugar, ejemplifica las distintas prioridades que tienen muchos chilenos. Comprensiblemente, algunos expresaron preocupación por la decisión del carabinero de realizar un disparo que, afortunadamente, sólo hirió en el brazo al conductor; cualquier error en su accionar hubiera podido costarle la vida al automovilista. Otros, también correctamente, han puesto el foco en la irresponsable decisión de este último, que desoyó las instrucciones del policía que le estaba apuntando con un arma; parece lógico pensar que, cuando alguien te apunta con un arma cargada, es mejor obedecer que arriesgar recibir un balazo. Por cierto, el insensato comunicado de la empresa Uber, que en ningún momento criticó la decisión de su “socio conductor” de desacatar la orden de un carabinero también generó justificado rechazo; ninguna empresa puede desconocer que sus trabajadores tienen la obligación de respetar la ley.

Pero más allá de la discusión sobre cómo se pudo haber evitado esa situación, el incidente mismo refleja un problema más profundo que existe en Chile. El conductor Uber optó por desafiar la autoridad de un carabinero, aun cuando éste le apuntaba con una pistola, porque el respeto a ellos parece una norma que se ha perdido. Vemos regularmente en marchas que los encapuchados —y muchos que ni siquiera se encapuchan— agreden violentamente a funcionarios que están haciendo su trabajo de proteger el orden público. Sabiendo que los que agreden a carabineros gozan de impunidad, muchas personas ni siquiera lo piensan dos veces antes de golpearlos o lanzarles piedras.

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