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Haemophilus species, as a urinary tract pathogen, is rarely encountered. 1‐3 Routine urine culture pathogens are well documented to include Enterobacteriaciae , Enterococcus spp., Staphylococcus spp., Pseudomonas spp., and Candida spp. Pathogens encountered rarely in this context may include Corynebacterium ureolyticus and nonfermenting gram negative bacilli. In this article, we present 2 case reports, one of Haemophilus influenzae in a 4-year-old Caucasian girl and the other of Haemophilus parinfluenzae in a 60-year-old African American man.

Case Reports

A urine culture on a 4-year-old Caucasian girl with a suspected urinary tract infection (UTI) grew 1 colony forming unit (CFU) per mL of Staphylococcus spp. and 2 other colonies after 12 hours of incubation. The plate was incubated overnight to ensure that the required 18-hour incubation period was met. The following day, there were pinpoint-sized colonies surrounding the Staphylococcus spp. colony ( Image 1 ). AGram stain was performed; the results showed those colonies to be made up of small gram negative bacilli. The colonies were subcultured to a chocolate agar plate (CHOC), which was incubated overnight in 5% CO 2 at 35°C. Also, the original blood agar plate (BLD) was streaked with the Staphyloccus spp. that had caused the satellite behavior (hereinafter, satelliting), in an attempt to see if additional behavior of this type could be visualized and a colony count could be estimated.

Image 1
Image 1
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Satelliting colonies on BLD agar plate from 4 year old female.

Image 1
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Satelliting colonies on BLD agar plate from 4 year old female.

After incubation, there was a small amount of additional growth adjacent to the Staphylococcus spp. streak. The original urine specimen had been discarded; as a result, direct plating to a CHOC for colony count was not possible. The CHOC grew colonies typical of Haemophilus spp. The colonies were identified as Haemophilus influenzae by the RapID NH kit (Thermo Fisher Scientific.) BBL Cefinase disc (Becton Dickinson and Company [BD]) testing yielded a negative result for beta lactamase production. A Kirby-Bauer susceptibility test was set up using BBL Haemophilus Test Media Agar (BD) and was incubated overnight in 5% CO 2 at 35°C. The susceptibility test yielded the following results: the organism was susceptible to ampicillin, azithromycin, cefaclor, cefuroxime, and trimethoprim sulfamethoxazole.

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